Extended and dynamic linker histone-DNA Interactions control chromatosome compaction
نویسندگان
چکیده
منابع مشابه
Linker Histone / DNA Interactions : In Vitro Studies
approved: Jordanka Zlatanova By studying the linker histone/DNA complex in vitro, our goal was to gain further insight into the interaction of these histones in chromatin structure, which, in turn, helps us in better understanding critical biological processes such as replication, transcription, and recombination. In direct competition experiments, we have confirmed previous results of the pref...
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Dynamic alterations in chromatin structure mediated by postsynthetic histone modifications and DNA methylation constitute a major regulatory mechanism in DNA functioning. DNA methylation has been implicated in transcriptional silencing, in part by inducing chromatin condensation. To understand the methylation-dependent chromatin structure, we performed atomic force microscope (AFM) studies of f...
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Linker histones are essential for DNA compaction in chromatin. They bind to nucleosomes in a 1:1 ratio forming chromatosomes. Alternative configurations have been proposed in which the globular domain of the linker histone H5 (gH5) is positioned either on- or off-dyad between the nucleosomal and linker DNAs. However, the dynamic pathways of chromatosome assembly remain elusive. Here, we studied...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2021
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2021.06.006